Daniel:Notebook/RNAFISH/2014-10-13
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ABP Trial Run (Started 10/10/2014)[edit]
Post Hybridization[edit]
- 2xSSPE/0.6% Triton X-100 2x 45mins. At room temp
- 1xHSB, 3x 30 mins at hybridization temperature
- Add 20 uL DAPI with Slow Fade Gold, cover with coverslip, seal with nail polish
- High Stringency Buffer:
- H2O 90ml
- 0.02M Na4P2O7 10ml
- 0.2M NaH2PO4 1.5ml