Daniel:Notebook/RNAFISH/2014-11-25

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RNA FISH-Cell Lines (Started 11/24/2014)[edit]

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Post Hybridization[edit]

Wash Version 2

  1. 2x 2xSSC 45 mins room temp
  2. 1x 0.2x SSC 45 mins hybridization temp
  3. Add 20 uL DAPI with Slow Fade Gold, cover with coverslip, seal with nail polish
  4. Wait >30 minutes to before imaging after applying nail polish

Microscopy[edit]

DNA FISH Comparison[edit]

I made the above images using auto contrast, which automatically sets the pixel range at the limits of the pixel values (usually just lowers the maximum value). To get a comparison, I then repeated the auto contrast function on some images from DNA FISH.