Daniel:Notebook/RNAFISH/2014-11-25
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RNA FISH-Cell Lines (Started 11/24/2014)[edit]
Post Hybridization[edit]
Wash Version 2
- 2x 2xSSC 45 mins room temp
- 1x 0.2x SSC 45 mins hybridization temp
- Add 20 uL DAPI with Slow Fade Gold, cover with coverslip, seal with nail polish
- Wait >30 minutes to before imaging after applying nail polish
Microscopy[edit]
- RNA FISH Cell Culture Test; RBFOX3/NeuN-488 (green); FOXP2-594 (red) columns=2
- 20141125 BE2C Series007 AutoContrast.jpg
BE2-C cells
- 20141125 NCIh1975.lif Series004 AutoContrast ch00.jpg
NCI h1975 cells
- 20141125 T98G.lif Series008 AutoContrast ch00.jpg
T98-G cells
- 20141125 U87MG.lif Series002 AutoContrast ch00.jpg
U98-MG
DNA FISH Comparison[edit]
I made the above images using auto contrast, which automatically sets the pixel range at the limits of the pixel values (usually just lowers the maximum value). To get a comparison, I then repeated the auto contrast function on some images from DNA FISH.
- DNA FISH 11/4/2014; DMD-488 (green); Cot1-546 (red) columns=2
- 20141105 SampleA.lif TileScan002 AutoContrast ch00.jpg
Sample A
- 20141105 SampleB.lif Series025 AutoContrast ch00.jpg
Sample B