Daniel:Notebook/RNAFISH/2014-9-3
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Trial Run (Started 08/29/2014)[edit]
Post-Hybidization[edit]
- Remove rubber cement together with the chamber using a pair of fine forceps
- Wash slides in 2X SSC at 37°C, 3 × 15 min (preferably with shaking) to remove hybridization mixture
- Perform stringency washes in 0.1X SSC at 60°C, 2 × 5 min to remove nonspecific hybrids
- Equilibrate in 2X SSC for 2 min
- Add DAPI, let sit for at least 30 minutes
- Seal the coverslip with nail polish; let sit 30 minutes
- Sections become fragile after the FISH; therefore, strong pressure on the coverslip should be avoided
- e.g., pressing the coverslip with the microscope objective when focusing often completely destroys the sample
Microscopy[edit]
This sample was viewed over several days, they are listed here:
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