Daniel:Notebook/RNAFISH/2015-3-6
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Linnarsson Lab Protocol 2 (Started 3/4/2015)[edit]
Post-Hybridization[edit]
- Warm up wash buffer and wash buffer with DAPI to 37C
- Rinse with wash buffer
- Add fresh was buffer and incubate for 30 min at 37C
- Replace with fresh wash buffer with DAPI and incubate for 30 min at 37C
- Wash 2 times with warm 2X SSC
- Mount the slide
Buffer Prep[edit]
- Wash Buffer
- 4 mL 20X SSC
- 4 mL Formamide
- 32 mL nfH2O
- Wash Buffer with DAPI
- 2 mL 20X SSC
- 2 mL Formamide
- 16 mL nfH2O
- 50 uL DAPI (100 ug/uL)