Daniel:Notebook/RNAFISH/2015-3-6

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Linnarsson Lab Protocol 2 (Started 3/4/2015)[edit]

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Post-Hybridization[edit]

  1. Warm up wash buffer and wash buffer with DAPI to 37C
  2. Rinse with wash buffer
  3. Add fresh was buffer and incubate for 30 min at 37C
  4. Replace with fresh wash buffer with DAPI and incubate for 30 min at 37C
  5. Wash 2 times with warm 2X SSC
  6. Mount the slide

Buffer Prep[edit]

  • Wash Buffer
    • 4 mL 20X SSC
    • 4 mL Formamide
    • 32 mL nfH2O
  • Wash Buffer with DAPI
    • 2 mL 20X SSC
    • 2 mL Formamide
    • 16 mL nfH2O
    • 50 uL DAPI (100 ug/uL)