Daniel:Protocols/CentriSep
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Centri-Sep Protocol[edit]
Princeton Separations Info: Centri Sep Protocol
Catalog No.
- CS-900 (32 pack) $109
- CS-901 (100 pack) $287
Use this protocol for Centri-Sep column purifications (Princeton Separations). This protocol uses size exclusion to separate DNA components from fluorophores post dye coupling.
Protocol[edit]
1. Hydrate in 800 uL ddH20; Allow at least 30 minutes for hydration, be sure to hydrate all the gel 2. Remove air bubbles by inverting and sharply tapping the column; stand up column and allow to settle 3. Drain the column into a wash tube (200-250 uL will drain) 4. Centrifuge at 750xg for 2 minutes (~3000 rpm; removes 300 uL fluid) 5. Transfer 20 uL reaction mixture to the top of the gel; do not disturb gel surface 6. Place column into collection tube and centrifuge at 750xg for 2 minutes 7. Dry sample in vacuum centrifuge