Daniel:Protocols/Qiaquick Column
Jump to navigation
Jump to search
Qiaquick Column Purification[edit]
Qiagen: Catalog #28104 (50) or #28106 (250)
Protocol[edit]
- Add 5X PB (binding buffer) to sample
- Load sample onto column and spin for 1 minute at 14000 rpm; discard flow through
- Add 750 uL PE (wash buffer) to column and spin for 1 minute at 14000 rpm; discard flow through
- Dry spin column for 1 minute at 14000 rpm; discard flow through
- Let stand with cover open in fume hood for ~5 minutes
- Transfer column to a new 1.5 mL eppendorf tube
- Add ≥30 uL EB to column
- Let stand 1 minute
- Spin for 1 minute at 14000 rpm