Daniel:Protocols/ssDNA Column
Jump to navigation
Jump to search
ssDNA Column Purification (Zymo)[edit]
Zymo: D7010 (20); D7011 (50)
Protocol[edit]
- Add 2 volumes Binding Buffer to the sample; mix well
- Transfer to IIC Column and centrifuge at 14000 rpm for 1 minute; SAVE THE FLOW THROUGH
- Add 1 volume 100% EtOH to flow through; mix well
- Transfer to IC Column and centrifuge at 14000 rpm for 1 minute; discard flow through
- Add 400 uL Prep Buffer and centrifuge at 14000 rpm for 1 minute; discard flow through
- Add 700 uL Wash Buffer and centrifuge at 14000 rpm for 1 minute; discard flow through
- Add 400 uL Wash Buffer and centrifuge at 14000 rpm for 1 minute; discard flow through
- Centrifuge empty column at 14000 rpm for 2 minutes
- Transfer to empty 1.5mL centrifuge tube (low bind)
- Add ≥ 6 uL nfH2O and centrifuge at 14000 rpm for 1 minute