Dinh:5hmC Quantification Samples Tracking
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Sample ID | Date | Treatment | Input amount (ng), post beads and post T4-BGT | Post-BS ssDNA (ng/uL) | Volume | Amount (ng) | % yield |
H1 p48 | 10/6/2015 | Bisulfite | 432 | 10.1 | 18 | 181.8 | 42% |
H1 p48 | 10/6/2015 | oxBS | 432 | 10.2 | 18 | 183.6 | 43% |
H1 p48 | 10/6/2015 | oxBS | 432 | 8.35 | 18 | 150.3 | 35% |
H1 p48 | 10/6/2015 | mTet1 (WiseGene) | 212 | 4.12 | 18 | 74.16 | 35% |
H1 p48 | 10/6/2015 | mTet1 (WiseGene) | 212 | 4.56 | 18 | 82.08 | 39% |
H1 p48 | 10/6/2015 | mTet2 (NEB) | 261 | 2.35 | 18 | 42.3 | 16% |
H1 p48 | 10/6/2015 | mTet2 (NEB) | 261 | 5.92 | 18 | 106.56 | 41% |
H1 p48 | 9/28/2015 | Bisulfite | 490 | 8.01 | 28 | 224.28 | 46% |
H1 p48 | 9/28/2015 | oxBS | 490 | 8.86 | 28 | 248.08 | 51% |
H1 p48 | 9/28/2015 | oxBS | 490 | 13.1 | 28 | 366.8 | 75% |
H1 p48 | 9/28/2015 | mTet1 (WiseGene) | 307 | 2.94 | 28 | 82.32 | 27% |
H1 p48 | 9/20/2015 | Bisulfite | 261 | 9.22 | 18 | 165.96 | 64% |
H1 p48 | 9/20/2015 | Bisulfite | 261 | 7.03 | 18 | 126.54 | 48% |
H1 p48 | 9/20/2015 | oxBS | 261 | 11.1 | 18 | 199.8 | 77% |
H1 p48 | 9/20/2015 | oxBS | 261 | 8.17 | 18 | 147.06 | 56% |
H1 p48 | 9/19/2015 | mTet1 (WiseGene) | 283 | 4.16 | 28 | 116.48 | 41% |