Dinh:Protocols/Bisulfite Capture
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- Nov 13 2009 Capturing - Practice, CpG30K, Positive control: Jurkat, Negative control: RNAse free-ddH20
- Improving capturing efficiency of CpG30K set for pipelining, Jan 4 2010 Capture Reaction (2pm), Jan 7 2010 Results.
Breast Cancer Patients, Diet and Methylation Study[edit]
- Capturing Breast Cancer Patients (samples 1-9), Dec 9 2009 Capture Reaction, Dec 15 2009 E-Gel Size selection, Amplification with iProof
- Breast Cancer patients (samples 10-19), Jan 6 2010 Bisulfite Conversion, Jan 7 2010 qPCR
- Breast Cancer patients (1-11, 20-28), Jan 12 2010 Bisulfite Conversion, qPCR_of_Bisulfite_Converted_DNA Jan 13 2010
Breast Cancer Patients (1-28) Samples Track, Dinh:Breast_Cancer_Methylation
- Capturing Test, CpG97A/B. Templates: bis_Jurkat, bis_16. Conditions: 55C vs 60C, 0 Cycling. Jan 10 2010
- Capture, CpG97A/B. Templates: bis_10-14, bis_Jurkat. Condition: 55C, 0 Cycling. Jan 15 2010
- Capture Re-test for Sanger Sequencing, CpG97A/B. Template: bis_15. Condition: 55C vs 60C, 0 Cycling. Jan 21 2010
- Capture PCR Reaction with AmpF/R6.3NH2, CpG97K A/B. Template: 10-15. Jan 25 2010, size selection Jan 26 2010, Re-Amplification Jan 27 2010, Samples sent to Illumina for Covaris shearing (all 100bp fragments) Jan 29 2010.
- Capture PCR Reaction with AmpF/R6.3NH2, CpG97A/B. Template: bis_16-28, jurkat. Condition: 55C annealing temp. 0 Cycling. Feb 8 2010, size selection Feb 12 2010, capture amplification with leftover product Feb 17 2010. E-gel size selection by Ida & samples sent to Harvard for Covaris shearing (all 100bp fragments), Feb 17 2010.