EricChu:LabNotesMDA/2013-10-10

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MDA Device v7 (PGP1 #19) with Tagmentation[edit]

Purpose: Testing lower PCR cycles (7 cycles --> 2 cycles) in adding adapters[edit]

  • One PGP1 cell was used in the v7 device.
  • The cell was lysed by ALS and observed under the microscope. There was no pumping in the v7 device.
  • ALS was used to push the cell content into the 24 compartments.
  • NS and MDA mastermix was loaded as performed previously.
  • EricChu:LabNotesMDA/2013-5-14 protocol
  • Reaction was done overnight for 15 hours at 30C.