EricChu:LabNotesMDA/2013-3-22

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Quantification of Mouse gDNA MDA in device by qPCR[edit]

  • Remainder: conditions of sample 1-16 had high Phi29 concentration and sample 17-24 had normal Phi29 concentration (see March 20 notes)


File:03222013qPCR.jpg


  • qPCR curve of B2a/b standard with samples below

File:03212013B2.jpg

  • qPCR curve of mSINE standard with samples below

File:03222013msine.jpg


  • Images of DNA clusters in different chambers
    • Chambers 15-16 used high Phi29 concentration; Chambers 17-18 used low Phi29 concentration
Chamber 15 Chamber 16
File:03222013ch15highPhi29.jpg File:03222013ch16highPhi29.jpg
Chamber 17 Chamber 18
File:03222013ch17lowPhi29.jpg File:03222013ch18lowPhi29.jpg