EricChu:LabNotesMDA/2013-3-8
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Differences in Surface to Volume Ratio in MDA Devices[edit]
- Vertical chamber always have a smaller surface to volume ratio
- Shorter chamber (or higher height) creates smaller surface to volume ratio
- In an article "Polymerase chain reaction in high surface-to-volume ratio SiO2 microstructures," it has explored the inactivation of polymerase in high surface-to-volume effect.(http://www.ncbi.nlm.nih.gov/pubmed/15538781)
Calculating S-to-V Ratio[edit]
- S-to-V ratio in Quake's sperm MDA device was very low (0.02um^-1). The ratio was higher in the pLOS single cell paper (0.08um^-1).
- S-to-V ratio in our MDA device was 0.21um^-1, which was an order higher.
- Another test device of ours was better in S-to-V, but still worse than any of Quake's device.
20um center (red layer) MDA device
File:RoundMDADevice.jpg
Vertical MDA chamber device (50um red layer)
File:Vertical Test Device No Ring.jpg