EricChu:LabNotesMDA/2013-9-17

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MDA Device v7 (PGP1 #15) with Tagmentation[edit]

  • The following samples were labeled as EC-PGP1-Dev7-index#,index#-Sep17 and given to Alan for sequencing.
  • They could be pooled into the same lane as they all have different index #s. 5 million reads were asked.

Size Selection[edit]

File:Sept17TBEgel1.jpg File:Sept17TBEgel2.jpg
File:Sept17TBEgel1cut.jpg File:Sept17TBEgel2cut.jpg

MDA Device v9 (PGP1 #17) with Tagmentation[edit]

  • Repeating the experiment of 9-15-2013
  • Device preparation was described in EricChu:LabNotesMDA/2013-5-20
  • MDA recipe was followed to the protocol in EricChu:LabNotesMDA/2013-5-14
  • Compare experiments of (1) v7 device with Tagmentation and (2) v9 device with Tagmentation
  • One PGP1 cell was used in this experiment.
  • Note 1: Valve above the mixing chamber was used. The valve was pumping every 5 seconds for one minute.
  • Note 2: After air pushed the ALS+cell, about 5 chambers were estimated empty (7 chambers were partially empty). They were then filled with ALS.
  • Note 3: Valve between ALS and NS was opened for 2 minutes before MDA mix was pushed into the MDA chambers.