EricChu:LabNotesMDA/2014-2-5
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qPCR Alu and Mito of MDA generated libraries on 1-30-2014[edit]
- These libraries were prepared by tagmentation.
- 1ul of each library was used in a 20ul qPCR reaction.
- 1ul 10ng human gDNA was used as control.
- Alu primers were labeled black and Mito primers were labeled red.
- Alu curves of both libraries on 1-30-2014 (labeled Hi and Lo) were amplified later than their corresponding Mito curves. However, as seen in gDNA, Alu should be amplified earlier than Mito.
- In this experiment, most of the DNA were coming from mitochondrial DNA rather than chromosomal DNA.