EricChu:LabNotesMDA/2014-3-11

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MDA of SW480 in v8 device (SW480#1)[edit]

  • all 25 samples were collected in ~2ul volume.
  • 1ul of each was used for tagmentation and 1ul was for CoRE.
  • TagPCR looked good
  • all sample pooled together --> EC SW480#1 Tag Mar12

File:03112014SW480TagPCR1.jpg File:03112014SW480TagPCR2.jpg

  • Right to Left: sample 1-10

File:03122014SW480Tag1-10.jpg

  • Right to Left: sample 11-20

File:03122014SW480Tag11-20.jpg

  • Left to Right: sample 21-25

File:03122014SW480Tag21-25.jpg

Chamber Alu (pg) Mito Mito/Alu
1 5,401.00 10,790.00 2.00
2 7,780.00 9,869.00 1.27
3 6,911.00 14,710.00 2.13
4 19,710.00 16,900.00 0.86
5 4,047.00 13,060.00 3.23
6 5,828.00 12,220.00 2.10
7 3,922.00 5,754.00 1.47
8 3,579.00 8,699.00 2.43
9 6,954.00 17,970.00 2.58
10 8,782.00 49,420.00 5.63
11 6,749.00 20,500.00 3.04
12 7,200.00 14,400.00 2.00
13 8,074.00 9,877.00 1.22
14 6,369.00 14,470.00 2.27
15 6,332.00 4,257.00 0.67
16 11,680.00 11,180.00 0.96
17 5,082.00 13,160.00 2.59
18 6,241.00 13,070.00 2.09
19 9,643.00 22,210.00 2.30
20 6,958.00 8,245.00 1.18
21 3,720.00 7,207.00 1.94
22 6,591.00 11,000.00 1.67
23 6,162.00 15,650.00 2.54
24 1,666.00 9,499.00 5.70
25 616.40 782.80 1.27
26 N/A N/A #VALUE!