EricChu:LabNotesMDA/2014-3-6
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PGP1#22 Tagmentation without Pol1[edit]
- finished bead purification of No Pol1 samples
- gel image of each sample
- Left to right: Sample 1-10
- Left to right: Sample 11-20
- Right to left: Sample 21-25 and neg control
- qPCR of Alu and Mito
File:03062014NoPolAlu.jpg File:03062014NoPolMito.jpg
- Readings of Alu and Mito
Chamber | Alu (pg) | Mito (pg) | Mito/Alu |
1 | 7,430.00 | 1,166.00 | 0.16 |
2 | 8,796.00 | 2,686.00 | 0.31 |
3 | 8,138.00 | 1,684.00 | 0.21 |
4 | 5,504.00 | 193.10 | 0.04 |
5 | 664.90 | 54.04 | 0.08 |
6 | 2,206.00 | 6,943.00 | 3.15 |
7 | 2,029.00 | 316.20 | 0.16 |
8 | 903.10 | 46.92 | 0.05 |
9 | 2,633.00 | 4,187.00 | 1.59 |
10 | 252.30 | 110.00 | 0.44 |
11 | 12,900.00 | 4,934.00 | 0.38 |
12 | 3,618.00 | 899.80 | 0.25 |
13 | 8,731.00 | 11,010.00 | 1.26 |
14 | 6,054.00 | 5,821.00 | 0.96 |
15 | 277.70 | 125.50 | 0.45 |
16 | 4,136.00 | 4,347.00 | 1.05 |
17 | 9,120.00 | 6,873.00 | 0.75 |
18 | 18,680.00 | 15,190.00 | 0.81 |
19 | 7,098.00 | 36,780.00 | 5.18 |
20 | 4,906.00 | 13,320.00 | 2.72 |
21 | 10,090.00 | 10,210.00 | 1.01 |
22 | 7,566.00 | 18,280.00 | 2.42 |
23 | 6,179.00 | 11,230.00 | 1.82 |
24 | 5,778.00 | 2,838.00 | 0.49 |
25 | 15,100.00 | 1,791.00 | 0.12 |
Neg Ctrl | 5,068.00 | 95.09 | 0.02 |
- The samples were pooled into 3 libraries based on values in Alu.
- Pool EC-PGP1#22 NoPol Lo Mar6: Index 53-58, 63 (samples 5-10 and 15)
- Pool EC-PGP1#22 NoPol M Mar6: Index 14, 52, 60, 64, 68, 72 (samples 4, 12, 16, 20, 24 and neg control)
- Pool EC-PGP1#22 NoPol Hi Mar6: Index 13, 49-51, 59, 31-21, 65-67, 69-71 (samples 1-3, 11, 12, 14, 17-19, 21-23, 25)