EricChu:LabNotesMDA/2014-3-6

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PGP1#22 Tagmentation without Pol1[edit]

  • finished bead purification of No Pol1 samples
  • gel image of each sample
  • Left to right: Sample 1-10

File:03062014NoPolGel1.jpg

  • Left to right: Sample 11-20

File:03062014NoPolGel2.jpg

  • Right to left: Sample 21-25 and neg control

File:03062014NoPolGel3.jpg

  • qPCR of Alu and Mito

File:03062014NoPolAlu.jpg File:03062014NoPolMito.jpg

  • Readings of Alu and Mito
Chamber Alu (pg) Mito (pg) Mito/Alu
1 7,430.00 1,166.00 0.16
2 8,796.00 2,686.00 0.31
3 8,138.00 1,684.00 0.21
4 5,504.00 193.10 0.04
5 664.90 54.04 0.08
6 2,206.00 6,943.00 3.15
7 2,029.00 316.20 0.16
8 903.10 46.92 0.05
9 2,633.00 4,187.00 1.59
10 252.30 110.00 0.44
11 12,900.00 4,934.00 0.38
12 3,618.00 899.80 0.25
13 8,731.00 11,010.00 1.26
14 6,054.00 5,821.00 0.96
15 277.70 125.50 0.45
16 4,136.00 4,347.00 1.05
17 9,120.00 6,873.00 0.75
18 18,680.00 15,190.00 0.81
19 7,098.00 36,780.00 5.18
20 4,906.00 13,320.00 2.72
21 10,090.00 10,210.00 1.01
22 7,566.00 18,280.00 2.42
23 6,179.00 11,230.00 1.82
24 5,778.00 2,838.00 0.49
25 15,100.00 1,791.00 0.12
Neg Ctrl 5,068.00 95.09 0.02
  • The samples were pooled into 3 libraries based on values in Alu.
  • Pool EC-PGP1#22 NoPol Lo Mar6: Index 53-58, 63 (samples 5-10 and 15)
  • Pool EC-PGP1#22 NoPol M Mar6: Index 14, 52, 60, 64, 68, 72 (samples 4, 12, 16, 20, 24 and neg control)
  • Pool EC-PGP1#22 NoPol Hi Mar6: Index 13, 49-51, 59, 31-21, 65-67, 69-71 (samples 1-3, 11, 12, 14, 17-19, 21-23, 25)