EricChu:LabNotesMDA/2014-3-7

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PGP1#22 Tagmentation with Pol1[edit]

  • Finished Tag PCR1 and PCR2.
  • Finished qPCR of Alu and Mito

File:03062014TagPCR1withPol1.jpg File:03062014TagPCR2withPol1.jpg

  • Left to Right: sample 1-10

File:03062014TagGel1.jpg

  • Left to right: sample 11-20

File:03062014TagGel2.jpg

  • left to right: sample 21-25 + neg control

File:03062014TagGel3.jpg File:03062014TagwithPol1Alu.jpg File:03062014TagwithPol1Mito.jpg

Chamber Alu Mito Mito/Alu
1 5,327.00 1,201.00 0.23
2 5,559.00 1,086.00 0.20
3 5,129.00 1,554.00 0.30
4 6,633.00 300.40 0.05
5 5,784.00 932.90 0.16
6 5,997.00 31,670.00 5.28
7 6,684.00 3,826.00 0.57
8 10,430.00 562.70 0.05
9 17,210.00 64,750.00 3.76
10 5,708.00 4,642.00 0.81
11 15,520.00 29,400.00 1.89
12 10,300.00 5,134.00 0.50
13 11,640.00 25,770.00 2.21
14 15,230.00 18,150.00 1.19
15 12,500.00 9,584.00 0.77
16 15,700.00 24,560.00 1.56
17 27,860.00 40,710.00 1.46
18 21,430.00 43,310.00 2.02
19 12,150.00 181,100.00 14.91
20 22,570.00 87,760.00 3.89
21 12,540.00 22,580.00 1.80
22 11,220.00 38,830.00 3.46
23 10,140.00 44,380.00 4.38
24 6,802.00 5,077.00 0.75
25 7,127.00 97.81 0.01
26 N/A 27.95 #VALUE!
  • Samples were pooled into 3 libraries according to the Alu reading
  • EC-PGP1#22 Tag Lo Mar7: Index 49-55, 58
  • EC-PGP1#22 Tag M Mar7: Index 56, 57, 59-64, 67, 69-71
  • Ec-PGP1#22 Tag Hi Mar7: Index 13, 65, 66, 68, 72

Library Verification[edit]

File:03072014PGP1.jpg