EricChu:LabNotesMDA/2014-3-7
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PGP1#22 Tagmentation with Pol1[edit]
- Finished Tag PCR1 and PCR2.
- Finished qPCR of Alu and Mito
File:03062014TagPCR1withPol1.jpg File:03062014TagPCR2withPol1.jpg
- Left to Right: sample 1-10
- Left to right: sample 11-20
- left to right: sample 21-25 + neg control
File:03062014TagGel3.jpg File:03062014TagwithPol1Alu.jpg File:03062014TagwithPol1Mito.jpg
Chamber | Alu | Mito | Mito/Alu |
1 | 5,327.00 | 1,201.00 | 0.23 |
2 | 5,559.00 | 1,086.00 | 0.20 |
3 | 5,129.00 | 1,554.00 | 0.30 |
4 | 6,633.00 | 300.40 | 0.05 |
5 | 5,784.00 | 932.90 | 0.16 |
6 | 5,997.00 | 31,670.00 | 5.28 |
7 | 6,684.00 | 3,826.00 | 0.57 |
8 | 10,430.00 | 562.70 | 0.05 |
9 | 17,210.00 | 64,750.00 | 3.76 |
10 | 5,708.00 | 4,642.00 | 0.81 |
11 | 15,520.00 | 29,400.00 | 1.89 |
12 | 10,300.00 | 5,134.00 | 0.50 |
13 | 11,640.00 | 25,770.00 | 2.21 |
14 | 15,230.00 | 18,150.00 | 1.19 |
15 | 12,500.00 | 9,584.00 | 0.77 |
16 | 15,700.00 | 24,560.00 | 1.56 |
17 | 27,860.00 | 40,710.00 | 1.46 |
18 | 21,430.00 | 43,310.00 | 2.02 |
19 | 12,150.00 | 181,100.00 | 14.91 |
20 | 22,570.00 | 87,760.00 | 3.89 |
21 | 12,540.00 | 22,580.00 | 1.80 |
22 | 11,220.00 | 38,830.00 | 3.46 |
23 | 10,140.00 | 44,380.00 | 4.38 |
24 | 6,802.00 | 5,077.00 | 0.75 |
25 | 7,127.00 | 97.81 | 0.01 |
26 | N/A | 27.95 | #VALUE! |
- Samples were pooled into 3 libraries according to the Alu reading
- EC-PGP1#22 Tag Lo Mar7: Index 49-55, 58
- EC-PGP1#22 Tag M Mar7: Index 56, 57, 59-64, 67, 69-71
- Ec-PGP1#22 Tag Hi Mar7: Index 13, 65, 66, 68, 72