EricChu:Qiagen MDA protocol

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Qiagen MDA protocol[edit]

  1. Prepare denaturation solution: 4.5ul DLB + 0.5ul 8% Tween20
  2. Add 1.4ul λ DNA to 1.4ul denaturation solution, incubate at room temp 5mins
  3. Add 1.4ul stop solution
  4. Create master mix (w/ 1% Tween20)
    1. 14.5ul Rxn Buffer
    2. 2.9ul 8% Tween20
    3. 1ul Phi29 polymerase
    4. 0.8ul H2O