EricChu:Qiagen MDA protocol
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Qiagen MDA protocol[edit]
- Prepare denaturation solution: 4.5ul DLB + 0.5ul 8% Tween20
- Add 1.4ul λ DNA to 1.4ul denaturation solution, incubate at room temp 5mins
- Add 1.4ul stop solution
- Create master mix (w/ 1% Tween20)
- 14.5ul Rxn Buffer
- 2.9ul 8% Tween20
- 1ul Phi29 polymerase
- 0.8ul H2O