Hosuk:LabNotes/2013-10-20
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Split cells in 96 well plate[edit]
Surface area of each culture dish[edit]
- T25 flask = 25 cm2
- MatTek dish = 1.54 cm2 (1.4cm diameter)
- 6 well plate = 9.5 cm2 --> 6 well : T25 flask = 1 : 2.6
- 96 well plate = 0.32 cm2 --> 96 well : MatTek dish = 1 : 4.8
Jonathan’s cell split[edit]
- He has cultured cells in 6 well plate.
- Add 500uL Trypsin, incubate
- Add 500uL media (total 1mL)
- Add 100uL media to each well in 96 well plate
- Add 60uL cell (from 1mL in 6 well plate) to each well in 96 well plate
What I did today is…[edit]
- Add 1mL Trypsin in T25 flask
- Add 4mL media (total 5mL)
- Add 100uL cell (from 5mL in T25 flask) to each well in 96 well plate (total 16 wells)
- Add 200uL media to each well, and gently mix
Rolonies with fresh cells - MatTek dish[edit]
- Produce rolonies in MatTek dishes which were fixed at 10/17, the same cells to 96 well plate.
Process[edit]
- 2 dishes
- RT : 8:30 pm
- CircLigase : 10/21 12:00 pm