Hosuk:LabNotes/2013-10-22

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Rolonies with fresh cells - MatTek dish[edit]

  • Produce rolonies in MatTek dishes which were fixed at 10/17, the same cells to 96 well plate.

Process[edit]

  • 2 dishes
  • RT : 10/20 8:30 pm
  • CircLigase : 10/21 12:00 pm
  • End RCA : 10/22 13:00pm
  • Imaging


Result[edit]

  • Good, many rolonies, and more ACTB than before.
    • Of course, the number and density of rolonies are still not as many as the sample 06/23...
  • Doing 1 location as well as tile scanning
    • Now Confocal is doing tile scanning...
    • Tile : 7 x 6 (1 tile field of view : 581.25um x 581.25um),
    • Total Z scan :35.81um, step : 1.23um, Total 30 stacks
    • 3 hr. 39min





  • Image processing and counting will be done with using PISA...