Hosuk:LabNotes/2013-10-22
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Rolonies with fresh cells - MatTek dish[edit]
- Produce rolonies in MatTek dishes which were fixed at 10/17, the same cells to 96 well plate.
Process[edit]
- 2 dishes
- RT : 10/20 8:30 pm
- CircLigase : 10/21 12:00 pm
- End RCA : 10/22 13:00pm
- Imaging
Result[edit]
- Good, many rolonies, and more ACTB than before.
- Of course, the number and density of rolonies are still not as many as the sample 06/23...
- Doing 1 location as well as tile scanning
- Now Confocal is doing tile scanning...
- Tile : 7 x 6 (1 tile field of view : 581.25um x 581.25um),
- Total Z scan :35.81um, step : 1.23um, Total 30 stacks
- 3 hr. 39min
- epi FL pictures (Zeiss, 20x NA=0.75, 400um x 400um, single z plane)
- File:S1 S2 MatTekDish epiFL 2013-10-22.png
- Confocal pictures
- location 1 : 1st Rolonies, ACTB
- location 2 : 1st Rolonies, ACTB
- Image processing and counting will be done with using PISA...