Hosuk:LabNotes/2013-10-4

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Rolonies 96well plate[edit]

Goal[edit]

  • Produce many rolony samples
  • Compare the result using my reagents, and new (Matt's) reagents


Process[edit]

  • Start at 10/02/13, end 4pm at 10/04/13
  • All 16 wells have been done RT process, and BS(PEG)9, but 4 wells went though whole process.
  • Imaging at 10/04/13, with 1st rolony probe and ACTB probe


Result[edit]

  • Matt's wells were not seen many rolonies, whereas mine have many.
  • I think the reason is white vial of a-dUTP, Matt used the white vial a-dUTP, and I used brown vial one.