Hosuk:LabNotes/2013-10-9

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1st Rolonies and ACTB Gene detection in 1st Rolonies[edit]

  • Tile scan, 2k x 2k resolution images with Confocal


Parameters[edit]

  • 488nm laser for ACTB target probe (ATTO488), 638nm for 1st Rolonies (Cy5)
  • 20x obj., zoom = 1.00, Pinhole = 2.00
  • Resolution format : 2048 x 2048 Format (8192 x 8192 --> Pixel size : 70.96nm x 70.96nm)
  • Field of view : 581.25um x 581.25um
  • Pixel size : 284nm x 284nm
  • PMT1 (ATTO488) : Gain 670V, Offset -2.00%
  • PMT2 (Cy5) : Gain 550V, Offset -2.00%
  • Z stack : step size : 0.54um, Total 10.87um scan (21 images)
  • Tile size = 8 x 6 --> 48 tiles
  • Total scanning time : 3hr. 10min


Result[edit]

  • Analysis is on going