Hosuk:LabNotes/2013-3-5

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Rolony construction and imaging[edit]

  • Completed sample #3 and #4
  • Imaging with Confocal
    • Much better than previous time
    • More number of rolonies (s#3)
  • However, there were fewer rolonies in s#4 (high conc. of phi29).
    • s#4 should have more rolonies than s#3 has.
    • why? maybe too old phi29?
  • Images : sample #3 (normal condition)
20x obj., Zoom = 1, 2048x2048 pixel 20x obj., Zoom = 1, 2048x2048 pixel
Cy3 channel (FOV: 581.25um x 581.25um) Combined(cell image+Cy3)
(Original) (Original)
File:Experiment S3 Img17 pos3 Cy3 20xObj z1 res2k.jpg File:Experiment S3 Img18 pos3 Cy3 20xObj z1 res2k Merged001.jpg


  • Images : sample #4 (normal condition)
20x obj., Zoom = 1, 2048x2048 pixel 20x obj., Zoom = 1, 2048x2048 pixel
Cy3 channel (FOV: 581.25um x 581.25um) Combined(cell image+Cy3)
(Original) (Original)
File:Experiment S4 Img25 pos2 Cy3 20xObj z1 res2k-1.jpg File:Experiment S4 Img25 pos2 Cy3 20xObj z1 res2k Merged001.jpg


  • Compare previous and this experiment

File:Compare 1st 2nd try.jpg