Hosuk:LabNotes/2013-3-5
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Rolony construction and imaging[edit]
- Completed sample #3 and #4
- Imaging with Confocal
- Much better than previous time
- More number of rolonies (s#3)
- However, there were fewer rolonies in s#4 (high conc. of phi29).
- s#4 should have more rolonies than s#3 has.
- why? maybe too old phi29?
- Images : sample #3 (normal condition)
20x obj., Zoom = 1, 2048x2048 pixel | 20x obj., Zoom = 1, 2048x2048 pixel | |
Cy3 channel (FOV: 581.25um x 581.25um) | Combined(cell image+Cy3) | |
(Original) | (Original) | |
File:Experiment S3 Img17 pos3 Cy3 20xObj z1 res2k.jpg | File:Experiment S3 Img18 pos3 Cy3 20xObj z1 res2k Merged001.jpg |
- Images : sample #4 (normal condition)
20x obj., Zoom = 1, 2048x2048 pixel | 20x obj., Zoom = 1, 2048x2048 pixel | |
Cy3 channel (FOV: 581.25um x 581.25um) | Combined(cell image+Cy3) | |
(Original) | (Original) | |
File:Experiment S4 Img25 pos2 Cy3 20xObj z1 res2k-1.jpg | File:Experiment S4 Img25 pos2 Cy3 20xObj z1 res2k Merged001.jpg |
- Compare previous and this experiment