Hosuk:LabNotes/2013-5-21
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Confocal imaging with the samples at 05/20[edit]
- Cy3-probes that I currently use seemed to be excited higher at 488nm than 552nm, which is weird.
- I've try lambda scanning to figure out where is emission spectrum range from the dye,
- and the emission spectrum was around 570-590nm which is Cy3 range.
- and the emission exited 488nm laser was stronger than 552nm.
- Very anti intuition though, but I could see rolonies in cells.
- And still there were too many big spots, which has to be removed!
- Need to wash more.
- I'll try normal protocol - from RT to RCA.
20x Oil, Position 3, Zoom = 1.00, resolution = 512 x 512[edit]
File:S4 Confocal 20xOil pos3 zoom1.00.png
20x Oil, Position 3, Zoom = 1.00, resolution = 2048 x 2048[edit]
File:S4 Confocal 20xOil pos3 zoom1.00 res2k 2.png
20x Oil, Position 6, Zoom = 3.50, resolution = 512 x 512[edit]
File:S4 Confocal 20xOil pos6 zoom3.50.png