Hosuk:LabNotes/2013-6-12
Jump to navigation
Jump to search
Day 3[edit]
- Jay finished last step, washing RCA and binding Cy3 probe, and image
- Rolonies are pretty well generated inside cells,
- and the signal is very strong, so there were almost no background
- Even though there were some of debris, they could be come from fixing step.
- No RCA signal were in -RT sample
20x, +RT vs. -RT (1 wash)[edit]
Raw images : +RT, -RT |
File:20x 1wash.png |
20x, +RT, NA=0.4 vs. 0.8 (2 wash)[edit]
Raw images : NA=0.4, NA=0.8 |
File:20x 2wash 0.4NA 0.8NA.png |
40x, +RT, NA=0.8, Cy3 vs. GFP channel (2 wash)[edit]
Raw images : Cy3, GFP |
File:40x 2wash 0.8NA Cy3 GFP.png |
63x, +RT, NA=1.2, Cy3 (2 wash)[edit]
Raw images : 63x |
File:63x 1.2NA 2wash 50msec 100EMgain ch00.png |