Hosuk:LabNotes/2013-6-21
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Rolony, 10th[edit]
- Use original volume and betaine from kit of CircLigase II reagents
- Start at 06/19
- Use cell dishes fixed at 06/19
- MetTak dish --> GC, lysine coated dish
- Run RT : 8:00pm 06/19 ~ 9:30am 06/20 --> ~13hr.
- Run CircLigase II : 12:30am 06/20 ~ 4:30pm 06/20 --> 4hr.
- Run RCA : 5:40 pm 06/20 ~ 1: 40pm 06/21 --> 20hr.
Result[edit]
- Similar to the result at 06/18
- low background, so I was able to image with 0.05s of exposure time, 50 EM Gain (previously 0.1s, 40EM gain)
- less dirt, background, but basically couldn't get many, dense rolonies
- Need to think about...