Hosuk:LabNotes/2013-6-21

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Rolony, 10th[edit]

  • Use original volume and betaine from kit of CircLigase II reagents


  • Start at 06/19
  • Use cell dishes fixed at 06/19
  • MetTak dish --> GC, lysine coated dish
  • Run RT : 8:00pm 06/19 ~ 9:30am 06/20 --> ~13hr.
  • Run CircLigase II : 12:30am 06/20 ~ 4:30pm 06/20 --> 4hr.
  • Run RCA : 5:40 pm 06/20 ~ 1: 40pm 06/21 --> 20hr.


Result[edit]

  • Similar to the result at 06/18
  • low background, so I was able to image with 0.05s of exposure time, 50 EM Gain (previously 0.1s, 40EM gain)
    • less dirt, background, but basically couldn't get many, dense rolonies
  • Need to think about...