Hosuk:LabNotes/2013-6-23

From ZhangLabWiki
Jump to navigation Jump to search

Rolony, 11th[edit]

  • S3 --> 3uL, S4 --> 10uL of Phi29
  • Use new vial of BS(PEG9) bought at 06/05


  • Start at 06/19
  • Use cell dishes fixed at 06/19
  • Fixing cells with 10% Formalin once (like Jonathan's cell dish)


  • Run RT : 9:00pm 06/23 ~ 9:00am 06/24 --> ~12hr.
  • Run CircLigase II : 1:00am 06/24 ~ 5:00pm 06/24 --> 4hr.
  • Run RCA : 6:10 pm 06/24 ~ 2: 10pm 06/25 --> 20hr.


Result[edit]

  • SUCCESS!!