Hosuk:LabNotes/2014-1-9

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RT primer annealing specific position in mRNA : 2nd try, in MatTek Dish[edit]

  • PGP1F P13 in MatTek Dish : #1 for RT Primer D220, #2 for RT Primer D1000 (01/07~01/09)


Result[edit]

  • 1st Rolony detection probe (Cy5) : count = 5135
  • RAB7A detection probe (ATTO550) : count = 64
  • Overlap count of RAB7A : count = 14
    • 'Almost no RAB7A target in 1st Rolony…WHY?…