Hosuk:LabNotes/2014-10-19
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Human Brain Tissue : fixing test[edit]
Result of Mouse Brain Tissue[edit]
- Sample : Mouse Brain, 10um thick, fixed at 4C for 12hr
- Permeabilization with 0.25% Triton X-100 in DEPC-treated H2O, incubated for Sample 1 : 15 min, Sample 2 : 30 min
- 200 ul 0.1% pepsin in 0.1 N HCl for S1 : 10min, S2 : 20 min at 37C
- There are many rolonies, this condition works well for mouse brain tissue.
Result of Human Brain Tissue[edit]
- Try Human Brain Tissue fixed with 4% Formaldehyde at 4C for 12hr.
- Continued from 2014-10-16
- There are too few rolonies, this condition doesn't work well for human brain tissue.