Hosuk:LabNotes/2014-10-19

From ZhangLabWiki
Jump to navigation Jump to search


Human Brain Tissue : fixing test[edit]

Result of Mouse Brain Tissue[edit]

  • Sample : Mouse Brain, 10um thick, fixed at 4C for 12hr
  • Permeabilization with 0.25% Triton X-100 in DEPC-treated H2O, incubated for Sample 1 : 15 min, Sample 2 : 30 min
  • 200 ul 0.1% pepsin in 0.1 N HCl for S1 : 10min, S2 : 20 min at 37C
  • There are many rolonies, this condition works well for mouse brain tissue.





Result of Human Brain Tissue[edit]

  • Try Human Brain Tissue fixed with 4% Formaldehyde at 4C for 12hr.
  • Continued from 2014-10-16


  • There are too few rolonies, this condition doesn't work well for human brain tissue.