Hosuk:LabNotes/2014-10-20
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Decoding PGP1F - Agi26k 20gap, 0gap, 0gap with Suppressor[edit]
- Decoding PGP1F with different Padlock Probes
- From 2014-10-15
Result[edit]
- Suppressor worked well
- Decoded genes were not many
- 20gap didn’t make 2nd Rolonies well
- For the next plan
- Decode again with PGP1F-0gap, and 0gap with suppressor, I need to get more number of detected features
- So, I'll re-generate 2nd Rolonies and decode again
Result - Image and Data[edit]
- 1st Rolonies - good in each dish
- File:DecodingPGP1F Agi0gap-0gapSupp-20gap 1stRolonies.png
- 2nd Rolony images by maximum projected with all 7 cycle images
- Not as many as I expected...
- Too few 2nd Rolony detected from 20gap
- Intensity Distribution
- File:DecodingPGP1F Agi0gap-0gapSupp-20gap ColorDistribution.png
- Comparison between data - previous decoding data(decoded at 09/05), current, FISSEQ from Jay, and Gene expression from Illumina
- File:DecodingPGP1F Agi0gap-0gapSupp-20gap CompareGeneData.png