Hosuk:LabNotes/2014-10-3

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Tissue Section : fixing test[edit]

  • Continued from 2014-10-01
  • Finish RCA, and imaging (10/03)


Result[edit]

  • S1 : Mouse brain tissue, 4hr fix, Pepsin for 20min --> No good
  • S3 : Mouse brain tissue, 2hr fix, Pepsin for 20min --> No Good
  • S4 : Mouse brain tissue, 2hr fix, Pepsin for 10min --> Good




Result-PGP1F[edit]

  • Just I've made 1st rolonies on a new PGP1F cells in order to check my reagent (CircLigase II and Phi29) are fine.
  • and it seems ok.


File:PGP1F RCA-2014-10-03 ResultImage 1.png