Hosuk:LabNotes/2014-2-18

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NCIH1975 : Mutation Detection : Data Analysis[edit]

  • Different method from what I've done at 2/9
  • Data from 2/3


Procedure[edit]

  • Make one image from all 8 images by Maximum intensity projection , and Run PISA
  • Extract location information from the BW image(--> mask image)
  • Look all 8 images based on the location info from mask image, Decode Rolony signals


  • Set threshold (9000 or 10000), and search range : 9 pixel or 11 pixel (--> 4, or 5 pixel from the center position)
    • Most of rolony signals have above 20000 intensity and backgound have below 7000~8000


Result[edit]

Compare prev. method and current method[edit]
  • Different counts and positions for each target

File:Compare Method1-0208 Method2-0217.png


Target counts at different parameters[edit]
  • Different Threshold and search range values generated different counts as well as different positions...
  • Which one would be correct?...



Composite picture of the Same Position (Pos 2), at each different parameters