Hosuk:LabNotes/2014-2-26

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PGP1F cultured with noEGF media[edit]

  • PGP1F were cultured in the media without using growth factor (DMEM + 10% FBS + 1% P/S)
  • Transferred to two MetTek dish (02/25/14)
  • Fixed cells (02/26/14), one with 4% Formalin, another one with 10% Formalin
  • Start RT (02/26/14) : ~ 10hr. (primer annealing time was 20min at room temp.)
  • Start Circligase II reaction (02/27/14) : ~3 hr., use 4uL emzyme
  • Start RCA reaction (02/27/14) : ~20hr. 500U enzyme from high concec. phi29