Hosuk:LabNotes/2014-3-28

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Cancer Cell : Rolony generation with new RT Primers[edit]

  • Dish 1 NCIH1975 : EGFR + BRAF + STOP PRIMER of both EGFR and BRAF
  • Dish 2 NCIH1975 : EGFR + BRAF and NO STOP PRIMER
  • Processing : 03/36 ~ 03/38


Result[edit]

  • Both dishes looks similar number of 1st Rolonies
  • 1st Rolonies are not as many as random hexamer though.
  • Correction (2014/4/2):Well 1 : no Stop primer, Well 2 : w/i Stop Primer

File:RolonyCount CancerNewPrimer20140328.png