Hosuk:LabNotes/2014-3-3

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PGP1F cultured with noEGF media : Result[edit]

  • Continued from 02/26
  • Detection – 1st Rolonies, ACTB, RAB7A and MALAT1 on 1sdt Rolonies


Result : Sample fixed with 4% Formalin[edit]

  • More mRNAs than before, as well as more 1st Rolonies
  • RAB7A were most, and ACTB, and MALAT1 were fewer than other two genes


Counts[edit]

File:Counts 2014-03-03.png

  • Counting was done after aligning


Images[edit]

File:Rolonies ConfocalImage 2014-03-03.png


Result : Sample fixed with 10% Formalin[edit]

  • More mRNAs than before, but fewer than the sample fixed with 4% Formalin
  • More even distribution, not localized at nucleus


Counts[edit]

File:Counts Formalin10 2014-03-04.png

  • Counting was done after aligning


Images[edit]

File:Rolonies Fomalin10 ConfocalImage 2014-03-04.png


  • Comparison

File:Counts Formalin4-10 2014-03-04.png