Hosuk:LabNotes/2014-3-3
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PGP1F cultured with noEGF media : Result[edit]
- Continued from 02/26
- Detection – 1st Rolonies, ACTB, RAB7A and MALAT1 on 1sdt Rolonies
Result : Sample fixed with 4% Formalin[edit]
- More mRNAs than before, as well as more 1st Rolonies
- RAB7A were most, and ACTB, and MALAT1 were fewer than other two genes
Counts[edit]
- Counting was done after aligning
Images[edit]
- Raw: 1st Rolony, ACTB, RAB7A, MALAT1
File:Rolonies ConfocalImage 2014-03-03.png
Result : Sample fixed with 10% Formalin[edit]
- More mRNAs than before, but fewer than the sample fixed with 4% Formalin
- More even distribution, not localized at nucleus
Counts[edit]
File:Counts Formalin10 2014-03-04.png
- Counting was done after aligning
Images[edit]
- Raw: 1st Rolony, ACTB, RAB7A, MALAT1
File:Rolonies Fomalin10 ConfocalImage 2014-03-04.png
- Comparison