Hosuk:LabNotes/2014-3-6

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Co-culture with PGP1F and NCIH1975 for Mutation Detection, and etc.[edit]

  • Purpose : check mutation detection from PGP1F (as negative control) with padlock probes for cancer genes (EGRF)
  • Cell deisity : 105 / mL
  • Transfer volume : 500uL
  • Media added: 500L
  • Total 20,000 cells per well, (10,000 of PGP1F + 10,000 NCIH1975 when mixed)


  • 24 well plate with glass-bottom
    • Glass part : 13mm in diameter

File:CoCulture 24wellPlate.png