Hosuk:LabNotes/2014-3-6
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Co-culture with PGP1F and NCIH1975 for Mutation Detection, and etc.[edit]
- Purpose : check mutation detection from PGP1F (as negative control) with padlock probes for cancer genes (EGRF)
- Cell deisity : 105 / mL
- Transfer volume : 500uL
- Media added: 500L
- Total 20,000 cells per well, (10,000 of PGP1F + 10,000 NCIH1975 when mixed)
- 24 well plate with glass-bottom
- Glass part : 13mm in diameter