Hosuk:LabNotes/2014-5-23
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Nuclear Staining with DRAQ5[edit]
- DRAQ5 : Excited by 638nm,
- Conc. 4uM
- Aspirate PBS in MatTek dish, add DRAQ5, incubate for 15min at RT, and image without washing step
- Sample : PGP1F (03/21/2014)
Result[edit]
- Problem : Permanent Staining, Cy3 Dye was gone after DRAQ5 staining
- File:DRAQ5 2014-05-23.png