Hosuk:LabNotes/2014-7-23
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Deconvolution test - Update[edit]
PSF Functions[edit]
- Generated by PSF Generator plug-in
- PSF_20x_4096x4096_best1st_256x256.tif
- PSF_63x_2048x2048_best1st_40x40.tif
- PSF_63xZoom4_2048x2048_best1st_128x128.tif.tif
Procedure for PISA[edit]
- Don't do medfilt2 when deconvoluted images are used.
- deconvblind Image --> mat2gray (for normalize the image for PISA run)
Matlab code (latest version[edit]
PISA Parameters (same as 07/18)[edit]
- PISA Param for 20x, 2048 x 2048
- Gaussian Std : 2
- Gaussian Upper : -2e-4
- area upper: 16
- area lower: 3
- axratio lower: 0.5
- circ upper: 1.6
- circ lower: 0.8
- perim conn: 8
- bkgmult lower: 4
- PISA Param for 20x, 4096 x 4096
- Gaussian Std : 2
- Gaussian Upper : -5e-4
- area upper: 50
- area lower: 6
- axratio lower: 0.5
- circ upper: 1.6
- circ lower: 0.8
- perim conn: 8
- bkgmult lower: 3
- PISA Param for 63x, 2048 x 2048
- Gaussian Std : 3
- Gaussian Upper : -2e-4
- area upper: 81
- area lower: 9
- axratio lower: 0.6
- circ upper: 1.6
- circ lower: 0.8
- perim conn: 8
- bkgmult lower: 4
- PISA Param for 63x Zoom 4, 2048 x 2048
- Gaussian Std : 5
- Gaussian Upper : -2e-5
- area upper: 1296
- area lower: 81
- axratio lower: 0.5
- circ upper: 1.6
- circ lower: 0.8
- perim conn: 8
- bkgmult lower: 4