Hosuk:LabNotes/2014-7-31

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Decoding for Mutation detection of Cancer Cell[edit]

  • Try decoding (07/30, 07/31)
  • With Two samples
    • S1_0715 : +Ampligase and +KlenTaq at 45C for 24hr.
    • S2_0715 : +Ampligase and +KlenTaq at 60C for 24hr.

Result[edit]

  • No signal… need to check again!
  • 1st Rolony signal was not detected.
  • Another sample, ampligase at 60C : also no signal, somehow 1st rolonies were gone…


  • I guess 1st Rolonies were unfold and gone because of degraded BS(PEG)9.
  • New BS(PEG)9 will be used for the next run!


Next Run[edit]

  • Transfer NCIH1975 to two MatTek dishes (07/31)