Hosuk:LabNotes/2014-7-31
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Decoding for Mutation detection of Cancer Cell[edit]
- Try decoding (07/30, 07/31)
- With Two samples
- S1_0715 : +Ampligase and +KlenTaq at 45C for 24hr.
- S2_0715 : +Ampligase and +KlenTaq at 60C for 24hr.
Result[edit]
- No signal… need to check again!
- 1st Rolony signal was not detected.
- Another sample, ampligase at 60C : also no signal, somehow 1st rolonies were gone…
- I guess 1st Rolonies were unfold and gone because of degraded BS(PEG)9.
- New BS(PEG)9 will be used for the next run!
Next Run[edit]
- Transfer NCIH1975 to two MatTek dishes (07/31)