Hosuk:LabNotes/2014-9-26

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HB Tissue : 1st Rolony[edit]

  • Two MatTek dishes : fixing for 20min, and 30min
  • 0.1% Pepsin for 15min
  • +adUTP
  • Apply Pap pen just before adding RT mix


Procedure[edit]

Start RT (9/24 11:30am ~ ) Start RCA : 9/25 (~9hr.) Image : (2014-09-25)


Result[edit]

  • Most of tissues are gone, and not many rolonies
  • Need to figure out what could be wrong on fixing step