Hosuk:LabNotes/2014-9-26
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HB Tissue : 1st Rolony[edit]
- Two MatTek dishes : fixing for 20min, and 30min
- 0.1% Pepsin for 15min
- +adUTP
- Apply Pap pen just before adding RT mix
Procedure[edit]
Start RT (9/24 11:30am ~ ) Start RCA : 9/25 (~9hr.) Image : (2014-09-25)
Result[edit]
- Most of tissues are gone, and not many rolonies
- Need to figure out what could be wrong on fixing step