Hosuk:LabNotes/2015-2-6
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Human Brain Tissue : CA12k_Nov2014 V4 & V7 : 1st Try[edit]
4th day[edit]
- Continued from 02/05
- 02/06
- For V4 & V7 samples: Wash with PBS once
- Prepare 4uL of BS(PEG)9 in 196uL PBS
- add 200uL BS(PEG)9 mix, incubate for 1hr at RT
- Wash with PBS once, add Tris pH 8.0, incubate for 30min
- Wash with PBS once
Imaging[edit]
- For 1st Rolony samples
- Prepare detection Probe : Final Conc. 1uM --> 1uL of 100uM Cy3-Adapt + 99uL (2xSSC + 30% Formamide)
- Preheat detection Probe at 75C for 5min
- Add to sample and incubate at RT for 10min
- Wash two times with 2x SSC
Result[edit]
- Not good!
- S3: 1st Rolony sample looks fair cell condition, but it has few signal
- Other 2 dishes (V4 and V7) got damaged…
- I’ll try more batch!