Hosuk:LabNotes/2015-3-31
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Optimal RCA Reaction Time[edit]
- Too long RCA reaction may produce larger size of rolonies, which cause difficulty of rolony detection within denser area.
- We need to reduce RCA reaction time to make proper size of rolonies.
Rolony generation[edit]
- Sample : PGP1F on MatTek dish - 6 dishes
- 2hr., 4hr., 6hr., 8hr., 12hr., and 20hr.
- 1st rolony generation
- Done and image at 03/31
Results[edit]
- 6hr (6.5hr) seems sufficient.
- More detail analysis - such as average rolony size, average intensity at each reaction time - will be followed.