Hosuk:LabNotes/2015-3-31

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Optimal RCA Reaction Time[edit]

  • Too long RCA reaction may produce larger size of rolonies, which cause difficulty of rolony detection within denser area.
  • We need to reduce RCA reaction time to make proper size of rolonies.


Rolony generation[edit]
  • Sample : PGP1F on MatTek dish - 6 dishes
    • 2hr., 4hr., 6hr., 8hr., 12hr., and 20hr.
  • 1st rolony generation


  • Done and image at 03/31
Results[edit]
  • 6hr (6.5hr) seems sufficient.
  • More detail analysis - such as average rolony size, average intensity at each reaction time - will be followed.


File:MIP RCATimeTest 2015-03-31 PGP1F 2.png