Hosuk:LabNotesMDA/2013-6-19

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MDA with PGP1F, 4th[edit]

  • MDA run for 8hr @06/19
  • 133mM ALS
  • Mixing pump --> shorter mixing time: 1sec period, 5min
    • Much less residue were seen under the valve area


  • Push ring mixer volume with air
    • Left 5~6 chamber were empty… why so many?
  • Verification of the amount of amplicon by qPCR : will be done soon
  • CoRE fragmentation was done @06/19


Samples[edit]
Chamber # Volume (uL) Mass (pg/uL) Total (pg)
1 5.05
2 5.42
3 5.36
4 5.08
5 6.87
6 5.46
7 5.42
8 5.24
9 5.22
10 4.80
11 5.34
12 5.15
13 4.25
14 5.01
15 4.83
16 4.58
17 5.93
18 5.74
19 5.19
20 5.40
21 6.02
22 4.93
23 5.43
24 4.60