Hosuk:LabNotesMDA/2013-6-19
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MDA with PGP1F, 4th[edit]
- MDA run for 8hr @06/19
- 133mM ALS
- Mixing pump --> shorter mixing time: 1sec period, 5min
- Much less residue were seen under the valve area
- Push ring mixer volume with air
- Left 5~6 chamber were empty… why so many?
- Verification of the amount of amplicon by qPCR : will be done soon
- CoRE fragmentation was done @06/19
Samples[edit]
Chamber # | Volume (uL) | Mass (pg/uL) | Total (pg) | ||||
1 | 5.05 | ||||||
2 | 5.42 | ||||||
3 | 5.36 | ||||||
4 | 5.08 | ||||||
5 | 6.87 | ||||||
6 | 5.46 | ||||||
7 | 5.42 | ||||||
8 | 5.24 | ||||||
9 | 5.22 | ||||||
10 | 4.80 | ||||||
11 | 5.34 | ||||||
12 | 5.15 | ||||||
13 | 4.25 | ||||||
14 | 5.01 | ||||||
15 | 4.83 | ||||||
16 | 4.58 | ||||||
17 | 5.93 | ||||||
18 | 5.74 | ||||||
19 | 5.19 | ||||||
20 | 5.40 | ||||||
21 | 6.02 | ||||||
22 | 4.93 | ||||||
23 | 5.43 | ||||||
24 | 4.60 | ||||||