Hosuk:LabNotesMDA/2013-7-22
Jump to navigation
Jump to search
MDA with PGP1F, 9th, Dev v9.1[edit]
- Use new device (v9.1)
- Same experiment to 07/18, but try to run MDA in ALS chamber after transferring solution into compartments.
Experiment[edit]
- NS valve didn’t work properly but the reaction was kept going. (8hr reaction)
Result[edit]
- Not bad, considering bad experiment condition
- Ch25 is the reaction in ALS chamber.
- The amount of amplicon was estimated 483.26pg/uL, which is 724.89pg in total.
- While transferring ALS+Cell solution into compartment with air, the remained solution was kept drying (evaporation by air)
- Thus using H2O would be better than air.