Hosuk:LabNotesMDA/2013-7-26
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MDA with PGP1F, 11th, Dev v9.1[edit]
- Use new device (v9.1)
- Same experiment to 07/18, but try pushing ALS+Cell into compartments with H2O, not air.
Experiment[edit]
- Start : 5:30pm 07/25, End : 8:30am 07/26 --> about 15hr reaction
- qPCR run for verification
- Extract just about 1.1 uL at each tube and run qPCR.
Result[edit]
- Good, considering bad experiment condition
- First(Left) two chambers had lower amplicons because of less template, but it’s better than pushing with air.
- Total amount of DNA estimated were lower than previous run (07/18),
- but the distribution of amount of DNAs was not bad even though no active mixing!
- The samples would have better fragment size because of not much sheer stress.
- It looked enough amount for tagmentation
- File:QPCR Result MDAv9.1 2013-07-26 fig1 QGraph Table.png
- File:QPCR Result MDAv9.1 2013-07-26 fig2 History.png
- File:QPCR Result MDAv9.1 2013-07-26 fig3 History2.png
Next Plan[edit]
- Run MDA, and do tagmentation for library preparation