Hosuk:LabNotesMDA/2013-7-26

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MDA with PGP1F, 11th, Dev v9.1[edit]

  • Use new device (v9.1)
  • Same experiment to 07/18, but try pushing ALS+Cell into compartments with H2O, not air.


Experiment[edit]

  • Start : 5:30pm 07/25, End : 8:30am 07/26 --> about 15hr reaction
  • qPCR run for verification
    • Extract just about 1.1 uL at each tube and run qPCR.


Result[edit]

  • Good, considering bad experiment condition
    • First(Left) two chambers had lower amplicons because of less template, but it’s better than pushing with air.
    • Total amount of DNA estimated were lower than previous run (07/18),
    • but the distribution of amount of DNAs was not bad even though no active mixing!
    • The samples would have better fragment size because of not much sheer stress.
    • It looked enough amount for tagmentation



Next Plan[edit]

  • Run MDA, and do tagmentation for library preparation