Hosuk:LabNotesMDA/2013-8-27
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MDA with PGP1F, 14th, Dev v9.1[edit]
- Use mineral oil in valve lines, Water in valves for cell capture and mineral oil for rest of valves
- 10hr reaction
Result[edit]
- Result : Not bad…but
- Pushing water doesn’t look good. Chromosome wasn’t pushed completely.
- Ch 1, 2, 3 had not enough template, and MDA product.
- Pushing air and run MDA in denature chamber…
File:QPCR Result MDAv9.1 2013-08-26 QTable.png File:QPCR Result MDAv9.1 2013-08-26 HistoryRecent.png
Next plan[edit]
- Tagmentation
- Use 3uL extraction.
- 2uL instead was much easier and precise due to the line in pipette tip (10uL tip).
- 2uL sample + 3uL ALS(400 uM KOH)+ 3uL NS