Hosuk:LabNotesMDA/2013-8-27

From ZhangLabWiki
Jump to navigation Jump to search


MDA with PGP1F, 14th, Dev v9.1[edit]

  • Use mineral oil in valve lines, Water in valves for cell capture and mineral oil for rest of valves
    • 10hr reaction


Result[edit]

  • Result : Not bad…but
    • Pushing water doesn’t look good. Chromosome wasn’t pushed completely.
    • Ch 1, 2, 3 had not enough template, and MDA product.
    • Pushing air and run MDA in denature chamber…


File:QPCR Result MDAv9.1 2013-08-26 QTable.png File:QPCR Result MDAv9.1 2013-08-26 HistoryRecent.png


Next plan[edit]

  • Tagmentation
    • Use 3uL extraction.
    • 2uL instead was much easier and precise due to the line in pipette tip (10uL tip).
    • 2uL sample + 3uL ALS(400 uM KOH)+ 3uL NS