Jeff:LabNotes/Microbiome/2009-2-25
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PBS/Tween 20 washing[edit]
The following protocol was used to allow water to spread more easily on the microwells
- Add 20 uL of Tween 20 to 180 uL H20 (10% dilution)
- Add 19.8 mL of PBS to this solution. Mix thoroughly by shaking. The solution should look soapy. This leads to a final dilution of .1% Tween 20.
- Wash microwell in solution for 5 min
- Remove microwell, dry out on kimwipe
- When dry, add silicon gasket and then 30 uL of water to a well to see how it spreads
Results[edit]
The water spread very well over the microwell. 30 uL seems to be enough of a volume to cover the entire square. Some of the water seeped through the silicon gasket. Futhermore, some of the microwell arrays peeled off.
Discussion[edit]
Next time, wait for the microwells to dry more to get a better seal with the gasket. Also, wash in solution for less time (2-3 min) so that the arrays do not peel off.