Jeff:LabNotes/Microbiome/2010-6-1

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Pipette Pulling Techniques[edit]

  • I want to get a tip around 20-30 um in size
  • This is possible by doing a one sided pull and then breaking the tip
  • The following settings were used with the P-97 in the basement: Velocity 140, Time 100, Pressure 200, Heat 740
  • The pipette was loaded and pull was pressed. When the pipette begins to move, hold one side and use a little force on the other side. This should create a very long and narrow pipette tip.
  • Now, use the tile to score and break
  • Several distances were used, but scoring about a quarter to a third back from the end of the tip creates an ideal size.

Images

File:6-1-10 30 um.jpeg 30 um tip

File:6-1-10 35 um.jpeg 35 um tip