Jeff:LabNotes/Midas Animation

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Matt's comments:
Really cool video and easy to understand! and all my comments here are just trying to be nit-picky

  • At 00:45 should the DNA be fragmented? If so, should the voice mention that?
  • Starting at 1:00 every time it says polymerase it sounds like "pall-mer-aze"
  • At 1:30 for the MDA, shouldn't the polymerases keep going and not get stuck?
  • At 2:24, why are the first 2 PCR tubes next to each other and all the others are organized every-other. It makes the first corner tube seem unique

Dinh's comments:

  • Very cool how the color of the solution changes to indicate new mixtures.
  • I agree with Matt about the weird pronunciation for polymerase.
  • Does not mention how the fluorescence get incorporated with new DNA.
  • Primer binding pattern seems non-random, although the primer sequences are different, should it be evenly spaced?

Noi's comments:

  • This is cool Jeff!!
  • I am not sure that you assume that the viewers are already known about what is this animation about based on the title of the animation. I just feel like there should be a key word or short sentence to represent what is this animation about before starting with "the process begin with the..."
  • The display of microwell is very nice and clear about the details.
  • During MDA, I think you may already notice about the length of primers in the 1st and 2nd round of amplification are different
  • During DNA synthesis, I think it would be great to see how dNTP and fluorescent marker are incorporated to the new DNA strand (at least there should be molecule of fluorophores in dsDNA.
  • Is it too much details to make the 4 different colors of 4 bases?
  • Not sure if I am right. After binding of 2nd primer to the new strand DNA, did DNA synthesis go to the wrong direction (3'->5')?