Jie:LabNotes/CpgSeq/2009-7-14
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genomic alleotype validatioon of GLIPR1L2 on GM12863,64,72~75[edit]
reaction system x6 H2O 20ul 120ul 2x Mastermix 25ul 150ul AmpF(10uM) 2ul 12ul AmpR(10uM) 2ul 12ul template 1ul/each from each sample Total 50ul 300ul 94C 2min -> (94C 45S -> 55C 45S -> 72C 45S) x 35 ->72C 3 min -> 15C hold.
primers: GLIPR1L2_gDNA_imprinted_F:5' - TGT GGC TAC TGC TAC TGG GTT CTA - 3' GLIPR1L2_gDNA_imprinted_R:5' - AGC ATG TGT TAG AGT GTC CGC AGT - 3'
File:20090714 genomic alleotype validatioon of GLIPR1L2 on GM12863,64,72~75.jpg20090714_genomic alleotype validatioon of GLIPR1L2 on GM12863,64,72~75
I purified the samples and sent to Genewiz to do the sanger sequencing.