Jie:LabNotes/CpgSeq/2009-7-14

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genomic alleotype validatioon of GLIPR1L2 on GM12863,64,72~75[edit]

reaction system                                                 x6     
H2O                                                  20ul      120ul    
2x Mastermix                                         25ul      150ul      
AmpF(10uM)                                           2ul       12ul       
AmpR(10uM)                                           2ul       12ul     
template                                      1ul/each from each sample
Total                                                50ul      300ul
94C 2min -> (94C 45S -> 55C 45S -> 72C 45S) x 35 ->72C 3 min -> 15C hold.
primers: 
GLIPR1L2_gDNA_imprinted_F:5' - TGT GGC TAC TGC TAC TGG GTT CTA - 3' 
GLIPR1L2_gDNA_imprinted_R:5' - AGC ATG TGT TAG AGT GTC CGC AGT - 3'

File:20090714 genomic alleotype validatioon of GLIPR1L2 on GM12863,64,72~75.jpg20090714_genomic alleotype validatioon of GLIPR1L2 on GM12863,64,72~75

I purified the samples and sent to Genewiz to do the sanger sequencing.